The 24-year-old Jamaican has confirmed his participation in the Diamond League event and will line up alongside World Indoor 60m champion Richard Kilty, Great Britain’s Adam Gemili and former world champion Kim Collins at Hampden Park on July 12.
Blake, who is still feeling his way back to action following several months on the sidelines, will make his debut appearance in the Scotland and he is likely to return to Glasgow two weeks later for the Commonwealth Games.
“After such a strong 2012, it was disappointing not to be able to continue that form into 2013,” said Blake.
“The competition in the Diamond League rivals the competition you get at major championships and it’s that challenge that motivates me.
“I know it won’t be easy, but I’m determined to show that ‘The Beast’ is back bigger and better.”
Double Olympic world champion Mo Farah, Olympic gold medallist Greg Rutherford and 400m world champion Christine Ohuruogu are also set to take part in the Glasgow event.